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Steps involved in the LT 50 freezing test. Seeds were nucleated with shaved ice at -2 °C and the temperature was subsequently lowered at the rate of 2 °C for one hour. Samples were removed at various temperatures, thawed overnight at 2 °C, and then placed into a <t>germination</t> chamber at 23 °C in the dark.
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plant growth chamber mlr 350t  (Sanyo)
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Steps involved in the LT 50 freezing test. Seeds were nucleated with shaved ice at -2 °C and the temperature was subsequently lowered at the rate of 2 °C for one hour. Samples were removed at various temperatures, thawed overnight at 2 °C, and then placed into a <t>germination</t> chamber at 23 °C in the dark.
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plant growth chamber  (Percival Scientific)
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Steps involved in the LT 50 freezing test. Seeds were nucleated with shaved ice at -2 °C and the temperature was subsequently lowered at the rate of 2 °C for one hour. Samples were removed at various temperatures, thawed overnight at 2 °C, and then placed into a <t>germination</t> chamber at 23 °C in the dark.
Plant Growth Chamber, supplied by Percival Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plant growth chamber - by Bioz Stars, 2026-07
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plant growth chamber  (Macquarie Bank)
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Macquarie Bank plant growth chamber
Steps involved in the LT 50 freezing test. Seeds were nucleated with shaved ice at -2 °C and the temperature was subsequently lowered at the rate of 2 °C for one hour. Samples were removed at various temperatures, thawed overnight at 2 °C, and then placed into a <t>germination</t> chamber at 23 °C in the dark.
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https://www.bioz.com/product/plant+growth+chamber/pmc13013159-57-17-23?v=Macquarie+Bank
Average 86 stars, based on 1 article reviews
plant growth chamber - by Bioz Stars, 2026-07
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plant growth chamber  (Conviron Inc)
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Conviron Inc plant growth chamber
Steps involved in the LT 50 freezing test. Seeds were nucleated with shaved ice at -2 °C and the temperature was subsequently lowered at the rate of 2 °C for one hour. Samples were removed at various temperatures, thawed overnight at 2 °C, and then placed into a <t>germination</t> chamber at 23 °C in the dark.
Plant Growth Chamber, supplied by Conviron Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plant+growth+chamber/pmc13037784-73-27-31?v=Conviron+Inc
Average 86 stars, based on 1 article reviews
plant growth chamber - by Bioz Stars, 2026-07
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plant growth chamber  (Sanyo)
86
Sanyo plant growth chamber
Steps involved in the LT 50 freezing test. Seeds were nucleated with shaved ice at -2 °C and the temperature was subsequently lowered at the rate of 2 °C for one hour. Samples were removed at various temperatures, thawed overnight at 2 °C, and then placed into a <t>germination</t> chamber at 23 °C in the dark.
Plant Growth Chamber, supplied by Sanyo, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plant+growth+chamber/pm41834090-170-7-10?v=Sanyo
Average 86 stars, based on 1 article reviews
plant growth chamber - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier

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Steps involved in the LT 50 freezing test. Seeds were nucleated with shaved ice at -2 °C and the temperature was subsequently lowered at the rate of 2 °C for one hour. Samples were removed at various temperatures, thawed overnight at 2 °C, and then placed into a germination chamber at 23 °C in the dark.

Journal: Frontiers in Plant Science

Article Title: Assessing seed characteristics for improved winter survival of late-fall-seeded lentils

doi: 10.3389/fpls.2026.1802566

Figure Lengend Snippet: Steps involved in the LT 50 freezing test. Seeds were nucleated with shaved ice at -2 °C and the temperature was subsequently lowered at the rate of 2 °C for one hour. Samples were removed at various temperatures, thawed overnight at 2 °C, and then placed into a germination chamber at 23 °C in the dark.

Article Snippet: Thawed seeds were then germinated in Petri dishes lined with hydrated triple Whatman #1 filter paper, without free-standing water, at a constant temperature of +23 °C in darkness in the germination chamber (Powers Scientific, Inc., DS33SD, Ontario) ( ).

Techniques: